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The Bacterial Flagellum

Methods and Protocols

Paperback Engels 2018 9781493983414
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.

Specificaties

ISBN13:9781493983414
Taal:Engels
Bindwijze:paperback
Uitgever:Springer New York

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Inhoudsopgave

<p>Preface…<br> Table of Contents…<br> Contributors…<br> <br> </p><p>Part I Flagellar Type III Protein Export, Assembly and Gene Regulation in Salmonella enterica</p><p>1.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Fuel of the Bacterial Flagellar Type III Protein Export Apparatus<br> Tohru Minamino, Miki Kinoshita and Keiichi Namba</p><p>2.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Interactions of Flagellar Structural Subunits with the Membrane Export Components Machinery<br> Lewis D.B. Evans, Paul M. Bergen, Owain J. Bryant and Gillian M. Fraser</p>3.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Fluorescent Microscopy Techniques to Study Hook Length Control and Flagella Formation<br> Marc Erhardt<p></p>4.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Coupling of Flagellar Gene Expression with Assembly in Salmonella enterica<br> Fabienne F.V. Chevance and Kelly T Hughes <p></p><p>5.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Dynamic Measures of Flagellar Gene Expression <br> Santosh Koirala and Christopher V. Rao</p><p>Part II Structure of the Bacterial Flagellum</p>6.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Purification and Characterization of the Bacterial Flagellar Basal Body from Salmonella enterica<br> Shin-Ichi Aizawa<p></p>7.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Design and Preparation of the Fragment Proteins of the Flagellar Components Suitable for X-Ray Crystal Structure Analysis <br> Katsumi Imada<p></p>8.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Structural Analysis of the Flagellar Component Proteins in Solution by Small Angle X-Ray Scattering<br> Lawrence K. Lee<p></p><p>9.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Structural Study of the Bacterial Flagellar Basal Body by Electron Cryomicroscopy and Image Analysis<br> Akihoro Kawamoto and Keiichi Namba</p><p>10.&nbsp; Structure of the MotA/B Proton Channel <br> Akio Kitao and Yasutaka Nishihara</p><p>11.&nbsp; Mechanism of Stator Assembly and Incorporation into the Flagellar Motor<br> Seiji Kojima</p><p>Part III Dynamics of the Bacterial Flagellar Motor</p><p>12.&nbsp; Rotation Measurements of Tethered Cells <br> Yuichi Inoue</p><p>13.&nbsp; Tracking the Movement of a Single Prokaryotic Cell in Extreme Environmental Conditions<br> Masayoshi Nishiyama and Yoshiyuki Arai</p><p>14.&nbsp; Measurements of the Rotation of the Flagellar Motor by Bead Assay<br> Taishi Kasai and Yoshiyuki Sowa</p><p>15.&nbsp; Measurements of Ion-Motive Force Across the Cell Membrane<br> Tsai-Shun Lin, Yi-Ren Sun, and Chien-Jung Lo</p><p>16.&nbsp; Stoichiometry and Turnover of the Stator and Rotor <br> Yusuke V. Morimoto and Tohru Minamino</p><p>17.&nbsp; Direct Imaging of Intracellular Signaling Molecule Responsible for the Bacterial Chemotaxis<br> Hajime Fukuoka</p>Part IV Structural Diversity of the Bacterial Flagellar Motors Derived from Different Bacterial Species<p></p><p>18.&nbsp; In Situ Structural Analysis of the Spirochetal Flagellar Motor by Cryo-Electron Tomography<br> Shiwei Zhu, Zhuan Qin, Juyu Wang, Dustin R. Morado, and Jun Liu</p><p>19.&nbsp; Motility of Spirochetes< Shuichi Nakamura and Md. Shafiqul Islam</p><p>20.&nbsp; Structure of the Sodium-Driven Flagellar Motor in Marine Vibrio <br> Yasuhiro Onoue and Michio Homma</p><p>21.&nbsp; Chemotactic Behaviors of Vibrio Cholerae Cells<br> Ikuro Kawagishi and So-ichiro Nishiyama</p><p>22.&nbsp; Purification of Fla2 Flagella of Rhodobacter sphaeroides<br> Javier de la Mora, Laura Camarena, and Georges Dreyfus</p><p>23.&nbsp; Dynamics in the Dual Fuel Flagellar Motor of Shewanella oneidensis MR-1<br> Susanne Brenzinger and Kai M. Thormann</p><p>24.&nbsp; Ion Selectivity of the Flagellar Motors Derived from the Alkaliphilic Bacillus and Paenibacillus Species<br> Yuka Takahashi and Masahiro Ito</p><p> </p><p>25.&nbsp; Measurement of Free-Swimming Motility and Magnetotactic Behavior of Magnetococcus massalia Strain MO-1<br> Wei-Jia Zhang, Sheng-Da Zhang, and Long-Fei Wu</p>

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