Tissue Proteomics

<p>Depletion of Myofibril-Associated Proteins Using Selective Protein Extraction as a Tool in Cardiac Proteomics.-&nbsp;Discovery of Immune Reactive Human Proteins by High Density Protein Arrays and Customized Validation of Potential Biomarkers by ELISA.-&nbsp;HLA Class I and Class II-Induced Intracellular Signaling and Molecular Associations in Primary Human Endothelial Cells.-&nbsp;Characterization of Protein Complexes Using Chemical Cross-linking Coupled Electrospray Mass Spectrometry.-&nbsp;Optimization for Peptide Sample Preparation for Urine Peptidomics.-&nbsp;Purification of Target Proteins from Native Tissues: CCT Complex from Bovine Testes and PP2Ac from Porcine Brains.-&nbsp;Integrative Analysis of Proteomics Data to Obtain Clinically Relevant Markers.-&nbsp;Untargeted Screening of Urinary Peptides Using Off-line Nano-liquid Chromatography – MALDI-TOF/TOF Mass Spectrometry.-&nbsp;Identifying Clinically Relevant Proteins for Targeted Analysis in the Development of a Multiplexed Proteomic Biomarker Assay.-&nbsp;In Situ Hybridization and Double Immunohistochemistry for the Detection of VEGF-A mRNA and CD34/Collagen IV Proteins in Renal Transplant Biopsies.-&nbsp;LC-SRM-based Targeted Quantification of Urinary Protein Biomarkers.-&nbsp;Fractionation of Soluble Proteins Using DEAE-, SP-, and Phenyl Sepharose Chromatographies for Proteomics.-&nbsp;GeLC-MS: A Sample Preparation Method for Proteomics Analysis of Minimal Amount of Tissue.-&nbsp;Targeted Proteomics Verification of Breast Cancer Biomarker Candidates.-&nbsp;Combination Strategy of Quantitative Proteomics Uncovers the Related-Proteins of Colorectal Cancer in the Interstitial Fluid of Colonic Tissue from the AOM-DSS Mouse Model.-&nbsp;Multiple Reaction Monitoring using Double Isotopologue Peptide Standards for Protein Quantification.-&nbsp;Isobaric Labeling Based LC-MS/MS Strategy for Comprehensive Profiling of Human Pancreatic Tissue Proteome.-&nbsp;MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the same Formalin-Fixed, Paraffin-embedded Tissue Section.-&nbsp;Differential Adipose Tissue Proteomics.-&nbsp;Quantification of Breast Cancer Protein Biomarkers at Different Expression Levels in Human Tumors.-&nbsp;Quantitative Proteomic Analysis of Mass Limited Tissue Samples for Spatially-resolved Tissue Profiling.-&nbsp;High-throughput Proteomics Analysis of Fresh-frozen Biopsy Tissue Samples using Pressure Cycling Technology Coupled with SWATH Mass Spectrometry.-&nbsp;Straightforward Protocol for Gel-free Proteomic Analysis of Adipose Tissue.-&nbsp;MALDI Imaging Combined to Laser Microdissection-based Microproteomics for Protein Identification. Application to Intratumor Heterogeneity Studies.</p>

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