Transgenic Maize
Methods and Protocols
Gebonden Engels 2009 2009e druk 9781934115497Samenvatting
As increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.
Specificaties
Lezersrecensies
Inhoudsopgave
1. Design of Gene Constructs for Transgenic Maize
Dong Liu
Part II: Transformation Methods
2. Transient Expression of GFP in Immature Seed Tissues
Colin T. Shepherd
3. Biolistic Gun Mediated Maize Genetic Transformation
Kan Wang and Bronwyn Frame
4. Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus
Vladimir Sidorov and David Duncan
5. Whiskers-Mediated Maize Transformation
Joseph F. Petolino and Nicole L. Arnold
Part III: Transgenic Maize in Research
6. Methods for Generation and Analysis of Fluorescent Protein Tagged Maize Lines
Amitabh Mohanty, Yan Yang, Anding Luo, Anne Sylvester, and David Jackson
7. Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression
Karen M. McGinnis
8. Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites
Guo-Ling Nan and Virginia Walbot
Part IV: Analysis of Transgenic Plants
9. Non-Radioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize
Guo-Ling Nan and Virginia Walbot
10. Tissue-Print Immuno-Detection of Transgene Products in Endosperm for High-Throughput Screening of Seeds
M. Paul Scott
11. Determination of Transgene Copy Number by Real-Time Quantitative-PCR
Colin T. Shepherd, Adrienne N. Moran Lauter, and M. Paul Scott
Part V: Breeding with Transgenes
12. Herbicide Resistance Screening Assay
Joan M. Peterson
13. Characterizing Transgene Inheritance
Earl H. Bicar
14. Backcross Breeding
Karla E. Vogel
Appendix A: Sample APHIS Notification of Release of Regulated Material
Appendix B: Sample SOP for Field Release
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