Annotating New Genes

From in Silico Screening to Experimental Validation

Gebonden Engels 2012 9781907568688
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

In recent years, a number of academic and commercial software packages and databases have been developed for the analysis and screening of biological data; however, the usability of these data is compromised by so-called novel genes to which no biological function is assigned. Annotating new genes outlines an approach to the analysis of evolutionary-conserved, heart-enriched genes with unknown functions, offering a step-by-step description of the procedure from screening to validation. The book begins by offering an introduction to the databases and software available, before moving on to cover programming guidelines, including a specific case study on the use of C-It for in silico screening. The second half of the book offers a step-by-step guide to experimental validation concepts and procedures, as well as an overview of additional potential applications of this approach in the field of stem cells and tissue regeneration, before a concluding chapter summarises the concepts and theories presented.

Specificaties

ISBN13:9781907568688
Taal:Engels
Bindwijze:Gebonden

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Inhoudsopgave

<p>List of figures and tables</p> <p>Acknowledgements</p> <p>About the author</p> <p>Chapter 1: Introduction</p> <p>Chapter 2: Databases and software to make your research life easier</p> <p>Abstract:</p> <p>2.1 Introduction</p> <p>2.2 Primary databases</p> <p>2.3 Secondary databases</p> <p>2.4 Datasets to keep an eye out for</p> <p>2.5 Conclusion</p> <p>Chapter 3: How to program like a bioinformatician?</p> <p>Abstract:</p> <p>3.1 Introduction</p> <p>3.2 Setting up an environment</p> <p>3.3 Writing Perl scripts</p> <p>3.4 Conclusion</p> <p>Chapter 4: Case study: C-It, knowledge database for screening evolutionarily conserved, tissue-enriched, uncharacterized genes</p> <p>Abstract:</p> <p>4.1 Introduction</p> <p>4.2 Concept of in silico screening</p> <p>4.3 Case study: C-It</p> <p>Chapter 5: Experimental validation: concepts and practice</p> <p>Abstract:</p> <p>5.1 Introduction</p> <p>5.2 Proposed experimental strategy</p> <p>5.3 Step 1: validation of expression patterns</p> <p>5.4 Step 2: phenotypic analysis</p> <p>5.5 Step 3: molecular interactions</p> <p>5.6 Other experiments</p> <p>5.7 Conclusion</p> <p>Chapter 6: Further application: adult stem cells and tissue regenerations</p> <p>Abstract:</p> <p>6.1 Introduction</p> <p>6.2 Cell types in heart and their lineages</p> <p>6.3 Current status of cardiac stem cells</p> <p>6.4 Conclusion</p> <p>Chapter 7: Conclusion</p> <p>Index</p>

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